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Alkaline Phosphatase Reporter Transposon for Identification of Genes Encoding Secreted Proteins in Gram-Positive Microorganisms
We describe the construction of TnFuZ, a genetic tool for the discovery and mutagenesis of proteins exported from gram-positive bacteria. This tool combines a transposable element (Tn4001) of broad host range in gram-positive bacteria and an alkaline phosphatase gene (phoZ) derived from a gram-posit...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology
2002
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC126685/ https://ncbi.nlm.nih.gov/pubmed/11823238 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.02.928-932.2002 |
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