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Alkaline Phosphatase Reporter Transposon for Identification of Genes Encoding Secreted Proteins in Gram-Positive Microorganisms

We describe the construction of TnFuZ, a genetic tool for the discovery and mutagenesis of proteins exported from gram-positive bacteria. This tool combines a transposable element (Tn4001) of broad host range in gram-positive bacteria and an alkaline phosphatase gene (phoZ) derived from a gram-posit...

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Detalles Bibliográficos
Publicado en:Appl Environ Microbiol
Principais autores: Gibson, Carmela M., Caparon, Michael G.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 2002
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC126685/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11823238/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.02.928-932.2002
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