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Y586F mutation in murine leukemia virus reverse transcriptase decreases fidelity of DNA synthesis in regions associated with adenine–thymine tracts

Using in vivo fidelity assays in which bacterial β-galactosidase or green fluorescent protein genes served as reporters of mutations, we have identified a murine leukemia virus (MLV) RNase H mutant (Y586F) that exhibited an increase in the retroviral mutation rate ≈5-fold in a single replication cyc...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Zhang, Wen-Hui, Svarovskaia, Evguenia S., Barr, Rebekah, Pathak, Vinay K.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2002
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC126629/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12119402/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.152186199
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