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Structure of the metal-independent restriction enzyme BfiI reveals fusion of a specific DNA-binding domain with a nonspecific nuclease

Among all restriction endonucleases known to date, BfiI is unique in cleaving DNA in the absence of metal ions. BfiI represents a different evolutionary lineage of restriction enzymes, as shown by its crystal structure at 1.9-Å resolution. The protein consists of two structural domains. The N-termin...

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Detalhes bibliográficos
Main Authors: Grazulis, Saulius, Manakova, Elena, Roessle, Manfred, Bochtler, Matthias, Tamulaitiene, Giedre, Huber, Robert, Siksnys, Virginijus
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2005
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1266039/
https://ncbi.nlm.nih.gov/pubmed/16247004
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0507949102
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