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Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate

Duplexes of 21–23 nucleotide (nt) RNAs are the sequence-specific mediators of RNA interference (RNAi) and post-transcriptional gene silencing (PTGS). Synthetic, short interfering RNAs (siRNAs) were examined in Drosophila melanogaster embryo lysate for their requirements regarding length, structure,...

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Autori principali: Elbashir, Sayda M., Martinez, Javier, Patkaniowska, Agnieszka, Lendeckel, Winfried, Tuschl, Thomas
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2001
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC125328/
https://ncbi.nlm.nih.gov/pubmed/11726523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/20.23.6877
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