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Site-Directed Mutagenesis and Expression of the Soluble Form of the Family IIIa Cellulose Binding Domain from the Cellulosomal Scaffolding Protein of Clostridium cellulovorans

The planar and anchoring residues of the family IIIa cellulose binding domain (CBD) from the cellulosomal scaffolding protein of Clostridium cellulovorans were investigated by site-directed mutagenesis and cellulose binding studies. By fusion with maltose binding protein, the family IIIa recombinant...

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Autors principals: Murashima, Koichiro, Kosugi, Akihiko, Doi, Roy H.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2005
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1251607/
https://ncbi.nlm.nih.gov/pubmed/16199585
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.20.7146-7149.2005
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