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Site-Directed Mutagenesis and Expression of the Soluble Form of the Family IIIa Cellulose Binding Domain from the Cellulosomal Scaffolding Protein of Clostridium cellulovorans

The planar and anchoring residues of the family IIIa cellulose binding domain (CBD) from the cellulosomal scaffolding protein of Clostridium cellulovorans were investigated by site-directed mutagenesis and cellulose binding studies. By fusion with maltose binding protein, the family IIIa recombinant...

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Autores principales: Murashima, Koichiro, Kosugi, Akihiko, Doi, Roy H.
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2005
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1251607/
https://ncbi.nlm.nih.gov/pubmed/16199585
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.187.20.7146-7149.2005
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