An engineered Escherichia coli tyrosyl–tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system

Tyrosyl–tRNA synthetase (TyrRS) from Escherichia coli was engineered to preferentially recognize 3-iodo-l-tyrosine rather than l-tyrosine for the site-specific incorporation of 3-iodo-l-tyrosine into proteins in eukaryotic translation systems. The wild-type TyrRS does not recognize 3-iodo-l-tyrosine...

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Bibliografische gegevens
Hoofdauteurs: Kiga, Daisuke, Sakamoto, Kensaku, Kodama, Koichiro, Kigawa, Takanori, Matsuda, Takayoshi, Yabuki, Takashi, Shirouzu, Mikako, Harada, Yoko, Nakayama, Hiroshi, Takio, Koji, Hasegawa, Yoshinori, Endo, Yaeta, Hirao, Ichiro, Yokoyama, Shigeyuki
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: The National Academy of Sciences 2002
Onderwerpen:
Biological Sciences
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC124990/
https://ncbi.nlm.nih.gov/pubmed/12097643
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.142220099
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