Enzymatic Characterization of Refolded Human Rhinovirus Type 14 2A Protease Expressed in Escherichia coli

Reported here is the production of recombinant human rhinovirus 14 (HRV14) 2A protease from bacterial cells transformed with a heat-inducible plasmid containing the HRV14 2A cDNA sequence. Overexpressed 2A protein partitioned into the inclusion bodies was solubilized in urea and then refolded in the...

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Détails bibliographiques

Publié dans:	J Virol
Auteurs principaux:	Wang, Q. May, Johnson, Robert B., Cox, Gregory A., Villarreal, Elcira C., Churgay, Lisa M., Hale, John E.
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1998
Sujets:	Animal Viruses
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC124656/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9445078/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.72.2.1683-1687.1998
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