A peptide-based fluorescence resonance energy transfer assay for Bacillus anthracis lethal factor protease

A fluorescence resonance energy transfer assay has been developed for monitoring Bacillus anthracis lethal factor (LF) protease activity. A fluorogenic 16-mer peptide based on the known LF protease substrate MEK1 was synthesized and found to be cleaved by the enzyme at the anticipated site. Extensio...

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Hauptverfasser: Cummings, Richard T., Salowe, Scott P., Cunningham, Barry R., Wiltsie, Judyann, Park, Young Whan, Sonatore, Lisa M., Wisniewski, Douglas, Douglas, Cameron M., Hermes, Jeffrey D., Scolnick, Edward M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: The National Academy of Sciences 2002
Schlagworte:
Biological Sciences
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC124449/
https://ncbi.nlm.nih.gov/pubmed/11997440
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.062171599
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