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A peptide-based fluorescence resonance energy transfer assay for Bacillus anthracis lethal factor protease
A fluorescence resonance energy transfer assay has been developed for monitoring Bacillus anthracis lethal factor (LF) protease activity. A fluorogenic 16-mer peptide based on the known LF protease substrate MEK1 was synthesized and found to be cleaved by the enzyme at the anticipated site. Extensio...
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Main Authors: | , , , , , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
The National Academy of Sciences
2002
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC124449/ https://ncbi.nlm.nih.gov/pubmed/11997440 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.062171599 |
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