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Identification of gene function by cyclical packaging rescue of retroviral cDNA libraries

Genes regulating responses in mammalian cells are often difficult to identify by functional cloning strategies limited to a single round of selection. Here we describe a strategy, cyclical packaging rescue (CPR), which allows rapid recovery and retransmission of retroviral cDNA libraries. CPR can be...

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Hlavní autoři: Bhattacharya, Deepta, Logue, Eric C., Bakkour, Sonia, DeGregori, James, Sha, William C.
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2002
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC124385/
https://ncbi.nlm.nih.gov/pubmed/12084928
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.132274799
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