Identification of gene function by cyclical packaging rescue of retroviral cDNA libraries

Genes regulating responses in mammalian cells are often difficult to identify by functional cloning strategies limited to a single round of selection. Here we describe a strategy, cyclical packaging rescue (CPR), which allows rapid recovery and retransmission of retroviral cDNA libraries. CPR can be...

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Bibliographic Details
Main Authors: Bhattacharya, Deepta, Logue, Eric C., Bakkour, Sonia, DeGregori, James, Sha, William C.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2002
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC124385/
https://ncbi.nlm.nih.gov/pubmed/12084928
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.132274799
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