Quantification of Uncultured Ruminococcus obeum-Like Bacteria in Human Fecal Samples by Fluorescent In Situ Hybridization and Flow Cytometry Using 16S rRNA-Targeted Probes

A 16S rRNA-targeted probe was designed and validated in order to quantify the number of uncultured Ruminococcus obeum-like bacteria by fluorescent in situ hybridization (FISH). These bacteria have frequently been found in 16S ribosomal DNA clone libraries prepared from bacterial communities in the h...

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Détails bibliographiques
Auteurs principaux: Zoetendal, Erwin G., Ben-Amor, Kaouther, Harmsen, Hermie J. M., Schut, Frits, Akkermans, Antoon D. L., de Vos, Willem M.
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2002
Sujets:
Microbial Ecology
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC124080/
https://ncbi.nlm.nih.gov/pubmed/12200269
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.9.4225-4232.2002
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