Quantification of 16S rRNAs in Complex Bacterial Communities by Multiple Competitive Reverse Transcription-PCR in Temperature Gradient Gel Electrophoresis Fingerprints

A novel approach was developed to quantify rRNA sequences in complex bacterial communities. The main bacterial 16S rRNAs in Drentse A grassland soils (The Netherlands) were amplified by reverse transcription (RT)-PCR with bacterium-specific primers and were separated by temperature gradient gel elec...

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Bibliografiska uppgifter
Huvudupphovsmän: Felske, Andreas, Akkermans, Antoon D. L., De Vos, Willem M.
Materialtyp: Artigo
Språk:Inglês
Publicerad: American Society for Microbiology 1998
Ämnen:
Methods
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC106687/
https://ncbi.nlm.nih.gov/pubmed/9797325
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