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Quantification of 16S rRNAs in Complex Bacterial Communities by Multiple Competitive Reverse Transcription-PCR in Temperature Gradient Gel Electrophoresis Fingerprints

A novel approach was developed to quantify rRNA sequences in complex bacterial communities. The main bacterial 16S rRNAs in Drentse A grassland soils (The Netherlands) were amplified by reverse transcription (RT)-PCR with bacterium-specific primers and were separated by temperature gradient gel elec...

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Udgivet i:Appl Environ Microbiol
Principais autores: Felske, Andreas, Akkermans, Antoon D. L., De Vos, Willem M.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1998
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Methods
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC106687/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9797325/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.11.4581-4587.1998
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