Noninvasive Measurement of Bacterial Intracellular pH on a Single-Cell Level with Green Fluorescent Protein and Fluorescence Ratio Imaging Microscopy

We show that a pH-sensitive derivative of the green fluorescent protein, designated ratiometric GFP, can be used to measure intracellular pH (pH(i)) in both gram-positive and gram-negative bacterial cells. In cells expressing ratiometric GFP, the excitation ratio (fluorescence intensity at 410 and 4...

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Bibliografische Detailangaben

Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Olsen, Katja N., Budde, Birgitte B., Siegumfeldt, Henrik, Rechinger, K. Björn, Jakobsen, Mogens, Ingmer, Hanne
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2002
Schlagworte:	Methods
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC123996/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12147523/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.8.4145-4147.2002
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