Noninvasive Measurement of Bacterial Intracellular pH on a Single-Cell Level with Green Fluorescent Protein and Fluorescence Ratio Imaging Microscopy

We show that a pH-sensitive derivative of the green fluorescent protein, designated ratiometric GFP, can be used to measure intracellular pH (pH(i)) in both gram-positive and gram-negative bacterial cells. In cells expressing ratiometric GFP, the excitation ratio (fluorescence intensity at 410 and 4...

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Detalhes bibliográficos
Main Authors: Olsen, Katja N., Budde, Birgitte B., Siegumfeldt, Henrik, Rechinger, K. Björn, Jakobsen, Mogens, Ingmer, Hanne
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC123996/
https://ncbi.nlm.nih.gov/pubmed/12147523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.8.4145-4147.2002
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