Characterization of a folding intermediate of human carbonic anhydrase II: probing local mobility by electron paramagnetic resonance.

The spin-labeling method was used to investigate human carbonic anhydrase, HCA II, undergoing unfolding induced by guanidine-HCI (Gu-HCI). The spin-probe, N-(2,2,5,5-tetramethyl-1-yloxypyrrolidinyl-3-yl)iodoacetamide, was attached covalently to the single cysteine (position 206) in the enzyme. The e...

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Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Lindgren, M, Svensson, M, Freskgård, P O, Carlsson, U, Jonasson, P, Mårtensson, L G, Jonsson, B H
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1995
Gaiak:
Research Article
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC1236238/
https://ncbi.nlm.nih.gov/pubmed/7669898
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