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Fluorescence spectral properties of troponin C mutant F22W with one-, two-, and three-photon excitation.

We report the first measurements of protein fluorescence with three-photon excitation, using a mutant of troponin C (TnC) that contains a single tryptophan residue F22W. From the emission intensity dependence on laser power we determine that TnC F22W displays one-, two-, and three-photon excitation...

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Main Authors: Gryczynski, I, Malak, H, Lakowicz, J R, Cheung, H C, Robinson, J, Umeda, P K
Formato: Artigo
Idioma:Inglês
Publicado: 1996
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1233832/
https://ncbi.nlm.nih.gov/pubmed/8968614
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