Protein–protein interactions monitored in mammalian cells via complementation of β-lactamase enzyme fragments

We have defined inactive α and ω fragments of β-lactamase that can complement to form a functional enzyme in both bacteria and mammalian cells, serving as a readout for the interaction of proteins fused to the fragments. Critical to this advance was the identification of a tripeptide, Asn-Gly-Arg, w...

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Publié dans:	Proc Natl Acad Sci U S A
Auteurs principaux:	Wehrman, Tom, Kleaveland, Benjamin, Her, Jeng-Horng, Balint, Robert F., Blau, Helen M.
Format:	Artigo
Langue:	Inglês
Publié:	National Academy of Sciences 2002
Sujets:	Biological Sciences
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC122547/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11904411/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.062043699
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