Protein–protein interactions monitored in mammalian cells via complementation of β-lactamase enzyme fragments
We have defined inactive α and ω fragments of β-lactamase that can complement to form a functional enzyme in both bacteria and mammalian cells, serving as a readout for the interaction of proteins fused to the fragments. Critical to this advance was the identification of a tripeptide, Asn-Gly-Arg, w...
Guardat en:
| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
2002
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC122547/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11904411/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.062043699 |
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