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Construction of a rationally designed human cytomegalovirus variant encoding a temperature-sensitive immediate-early 2 protein
We generated a set of cysteine-to-glycine mutations and screened them to identify a temperature-sensitive allele of the human cytomegalovirus UL122 gene, which encodes the immediate-early 2 transcriptional activating protein. The mutant allele contains a single base pair substitution at amino acid 5...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
2002
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC122486/ https://ncbi.nlm.nih.gov/pubmed/11867756 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.052710599 |
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