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Construction of a rationally designed human cytomegalovirus variant encoding a temperature-sensitive immediate-early 2 protein

We generated a set of cysteine-to-glycine mutations and screened them to identify a temperature-sensitive allele of the human cytomegalovirus UL122 gene, which encodes the immediate-early 2 transcriptional activating protein. The mutant allele contains a single base pair substitution at amino acid 5...

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Autors principals: Heider, Julie A., Bresnahan, Wade A., Shenk, Thomas E.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2002
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC122486/
https://ncbi.nlm.nih.gov/pubmed/11867756
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.052710599
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