Isomerization of the uncomplexed actinidin molecule: kinetic accessibility of additional steps in enzyme catalysis provided by solvent perturbation.

The effects of increasing the content of the aprotic dipolar organic co-solvent acetonitrile on the observed first-order rate constant (k(obs)) of the pre-steady state acylation phases of the hydrolysis of N-acetyl-Phe-Gly methyl thionester catalysed by the cysteine proteinase variants actinidin and...

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Main Authors: Reid, James D, Hussain, Syeed, Bailey, Tamara S F, Sonkaria, Sanjiv, Sreedharan, Suneal K, Thomas, Emrys W, Resmini, Marina, Brocklehurst, Keith
Formato: Artigo
Idioma:Inglês
Publicado: 2004
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1223986/
https://ncbi.nlm.nih.gov/pubmed/14640975
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20031318
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