Variation in the pH-dependent pre-steady-state and steady-state kinetic characteristics of cysteine-proteinase mechanism: evidence for electrostatic modulation of catalytic-site function by the neighbouring carboxylate anion.

The acylation and deacylation stages of the hydrolysis of N -acetyl-Phe-Gly methyl thionoester catalysed by papain and actinidin were investigated by stopped-flow spectral analysis. Differences in the forms of pH-dependence of the steady-state and pre-steady-state kinetic parameters support the hypo...

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Main Authors: Hussain, Syeed, Pinitglang, Surapong, Bailey, Tamara S F, Reid, James D, Noble, Michael A, Resmini, Marina, Thomas, Emrys W, Greaves, Richard B, Verma, Chandra S, Brocklehurst, Keith
Format: Artigo
Language:Inglês
Published: 2003
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1223443/
https://ncbi.nlm.nih.gov/pubmed/12643810
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20030177
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