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Transgenesis by lentiviral vectors: Lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos

The introduction of foreign genes into early mouse embryos and embryonic stem (ES) cells is invaluable for the analysis of gene function and regulation in the living animal. The use of vectors derived from retroviruses as gene transfer vehicles in this setting has had limited success because of sile...

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Autori principali: Pfeifer, Alexander, Ikawa, Masahito, Dayn, Yelena, Verma, Inder M.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The National Academy of Sciences 2002
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC122332/
https://ncbi.nlm.nih.gov/pubmed/11854510
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.251682798
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