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Mutation of the important Tyr-33 residue of chicken avidin: functional and structural consequences.
The strong interaction between avidin and biotin is so tight (dissociation constant 10(-15) M) that conditions usually sufficient for protein denaturing fail to dislodge biotin from the avidin-biotin complex. This kind of irreversible binding hinders the use of avidin in applications such as affinit...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1223082/ https://ncbi.nlm.nih.gov/pubmed/12358604 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20020886 |
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