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Denaturing action of urea and guanidine hydrochloride towards two thermophilic esterases.
The stability of two thermophilic esterases, AFEST from Archaeoglobus fulgidus and EST2 from Alicyclobacillus acidocaldarius, against the denaturing action of urea and guanidine hydrochloride has been investigated by means of steady-state fluorescence and circular dichroism measurements. Experimenta...
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| Main Authors: | , , , , , , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2002
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1222942/ https://ncbi.nlm.nih.gov/pubmed/12160466 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20020695 |
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