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Optimizing bioconversion pathways through systems analysis and metabolic engineering

We demonstrate a general approach for metabolic engineering of biocatalytic systems comprising the uses of a chemostat for strain improvement and radioisotopic tracers for the quantification of pathway fluxes. Flux determination allows the identification of target pathways for modification as valida...

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Detalhes bibliográficos
Main Authors: Stafford, Daniel E., Yanagimachi, Kurt S., Lessard, Philip A., Rijhwani, Sushil K., Sinskey, Anthony J., Stephanopoulos, Gregory
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC122274/
https://ncbi.nlm.nih.gov/pubmed/11854482
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.032681699
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