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Continuous affinity-based selection: rapid screening and simultaneous amplification of bacterial surface-display libraries.

A new method for continuous biopanning has been developed. We have combined the power of affinity chromatography with the fecundity of bacteria in a unique process that mimics clonal selection. Mixed populations of bacteria were applied to a fermenter containing the immobilized ligand of interest. B...

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Autors principals: Patel, D, Vitovski, S, Senior, H J, Edge, M D, Hockney, R C, Dempsey, M J, Sayers, J R
Format: Artigo
Idioma:Inglês
Publicat: 2001
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1222007/
https://ncbi.nlm.nih.gov/pubmed/11463348
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