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Purification and characterization of autophagosomes from rat hepatocytes.

To investigate the properties and intracellular origin of autophagosomes, a procedure for the purification and isolation of these organelles from rat liver has been developed. Isolated hepatocytes were incubated with vinblastine to induce autophagosome accumulation; the cells were then homogenized a...

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Detalhes bibliográficos
Main Authors: Strømhaug, P E, Berg, T O, Fengsrud, M, Seglen, P O
Formato: Artigo
Idioma:Inglês
Publicado em: 1998
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1219772/
https://ncbi.nlm.nih.gov/pubmed/9761717
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