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Quantification of cathepsins B and L in cells.
A method for quantifying active cysteine proteinases in mammalian cells has been developed using an active-site-directed inhibitor. Fluoren-9-ylmethoxycarbonyl(di-iodotyrosylalanyl)-diaz omethane (Fmoc-[I2]Tyr-Ala-CHN2) was prepared and shown to react irreversibly with cathepsins B and L, but not wi...
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Auteurs principaux: | , , |
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Format: | Artigo |
Langue: | Inglês |
Publié: |
1998
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Sujets: | |
Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1219506/ https://ncbi.nlm.nih.gov/pubmed/9601080 |
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