Reconstitution of native-like nucleosome core particles from reversed-phase-HPLC-fractionated histones.

We have reconstituted nucleosome core particles from reversed-phase-HPLC-purified chicken erythrocyte core histones and 145 bp random-sequence DNA fragments. Characterization of the resulting nucleoprotein complexes by sedimentation velocity, CD and DNase I footprinting showed that they are structur...

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Dettagli Bibliografici
Autori principali: Moore, S C, Rice, P, Iskandar, M, Ausió, J
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1997
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1218935/
https://ncbi.nlm.nih.gov/pubmed/9371695
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