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Expression, purification, and characterization of recombinant human glutamine synthetase.

A bacterial expression system has been engineered for human glutamine synthetase (EC 6.3.1.2) that produces approximately 60 mg of enzyme (20% of the bacterial soluble protein) and yields approx. 8 mg of purified enzyme per litre of culture. The recombinant enzyme was purified 5-fold to apparent hom...

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Bibliografske podrobnosti
Main Authors: Listrom, C D, Morizono, H, Rajagopal, B S, McCann, M T, Tuchman, M, Allewell, N M
Format: Artigo
Jezik:Inglês
Izdano: 1997
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC1218900/
https://ncbi.nlm.nih.gov/pubmed/9359847
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