Biophysical and mutagenic analysis of Thermoanaerobacter ethanolicus secondary-alcohol dehydrogenase activity and specificity.

The Thermoanaerobacter ethanolicus 39E adhB gene encoding the secondary-alcohol dehydrogenase (secondary ADH) was overexpressed in Escherichia coli at more than 10% of total protein. The recombinant enzyme was purified in high yield (67%) by heat-treatment at 85 degrees C and (NH4)2SO4 precipitation...

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Autores principales: Burdette, D S, Secundo, F, Phillips, R S, Dong, J, Scott, R A, Zeikus, J G
Formato: Artigo
Lenguaje:Inglês
Publicado: 1997
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1218725/
https://ncbi.nlm.nih.gov/pubmed/9307020
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