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Crystallization and preliminary X-ray diffraction analysis of the Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase I86A mutant

The Thermoanaerobacter ethanolicus secondary alcohol dehydrogenase I86A mutant is stereospecific for (R)-alcohols instead of (S)-alcohols. Pyramidal crystals grown in the presence of (R)-phenylethanol via the hanging-drop vapour-diffusion method diffracted to 3.2 Å resolution at the Canadian Light S...

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Detalhes bibliográficos
Main Authors: Protsko, Carla, Vieille, Claire, Laivenieks, Maris, Prasad, Lata, Sanders, David A. R., Delbaere, Louis T. J.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2898473/
https://ncbi.nlm.nih.gov/pubmed/20606285
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110018981
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