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Expression and mutagenesis of the catalytic domain of cGMP-inhibited phosphodiesterase (PDE3) cloned from human platelets.
We have used reverse transcriptase PCR, platelet mRNA and degenerate primers based on platelet peptide sequences, to amplify a fragment of platelet cGMP-inhibited phosphodiesterase (cGI-PDE; PDE3). Sequence analysis of this clone established that both the platelet and the cardiac forms of PDE3 were...
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Main Authors: | , , |
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Format: | Artigo |
Sprog: | Inglês |
Udgivet: |
1997
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Fag: | |
Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1218297/ https://ncbi.nlm.nih.gov/pubmed/9173884 |
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