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Two variants of quantitative reverse transcriptase PCR used to show differential expression of alpha-, beta- and gamma-fibrinogen genes in rat liver lobes.

Quantitative reverse transcriptase PCR (RT-PCR) is a sensitive method for the measurement of mRNA copy number. However, the methodology has gained a reputation for poor reproducibility, leading to concern over the validity of much of the data generated using this technique. We have developed two var...

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Detalhes bibliográficos
Main Authors: Zhang, J, Desai, M, Ozanne, S E, Doherty, C, Hales, C N, Byrne, C D
Formato: Artigo
Idioma:Inglês
Publicado em: 1997
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1218134/
https://ncbi.nlm.nih.gov/pubmed/9032465
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