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The Vacuolar Atpase of Neurospora Crassa Is Indispensable: Inactivation of the Vma-1 Gene by Repeat-Induced Point Mutation
To analyze the phenotype of cells lacking the vacuolar ATPase, we inactivated the vma-1 gene, which encodes the catalytic subunit of the enzyme. Because preliminary experiments suggested the vma-1 gene was essential, we developed a method of simultaneously inactivating the gene and complementing it...
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| Auteurs principaux: | , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
1996
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1207248/ https://ncbi.nlm.nih.gov/pubmed/8722770 |
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