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Transformation of Yeast by Agitation with Glass Beads

We have found that agitation of Saccharomyces cerevisiae with glass beads and plasmid DNA using a vortex mixer results in genetic transformation of the yeast cells. This method is less efficient, but considerably more convenient, than other yeast transformation procedures. The fact that the minimal...

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Autors principals: Costanzo, M. C., Fox, T. D.
Format: Artigo
Idioma:Inglês
Publicat: 1988
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1203545/
https://ncbi.nlm.nih.gov/pubmed/3066683
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