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Glass bead transformation method for gram-positive bacteria

A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into protoplasts of several strains o...

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Hlavní autoři: Rattanachaikunsopon, Pongsak, Phumkhachorn, Parichat
Médium: Artigo
Jazyk:Inglês
Vydáno: Sociedade Brasileira de Microbiologia 2009
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3768579/
https://ncbi.nlm.nih.gov/pubmed/24031442
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1590/S1517-838220090004000025
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