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Mapping of Escherichia Coli Chromosomal Tn5 and F Insertions by Pulsed Field Gel Electrophoresis

A low resolution Not I physical map of Escherichia coli was recently constructed. In this report we demonstrated that this map can be used to map Tn5 and F insertions physically. The transposon, Tn5, contains Not I recognition sequences in its IS50 sequences. F plasmid contains an unmapped Not I sit...

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Bibliographic Details
Main Authors: Smith, C. L., Kolodner, R. D.
Format: Artigo
Language:Inglês
Published: 1988
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1203407/
https://ncbi.nlm.nih.gov/pubmed/2840334
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