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A Tn 10-lacZ-kanR-URA3 Gene Fusion Transposon for Insertion Mutagenesis and Fusion Analysis of Yeast and Bacterial Genes

We describe here a new variant of transposon Tn 10 especially adapted for transposon analysis of cloned yeast genes; it can equally well be used for analysis of prokaryotic genes. We have applied this element to analysis of the LEU2, RAD50, and CDC48 genes of Saccharomyces cerevisiae. This transposo...

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Detalhes bibliográficos
Main Authors: Huisman, Olivier, Raymond, Wendy, Froehlich, Kai-Uwe, Errada, Patrick, Kleckner, Nancy, Botstein, David, Hoyt, M. Andrew
Formato: Artigo
Idioma:Inglês
Publicado em: 1987
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1203129/
https://ncbi.nlm.nih.gov/pubmed/3038670
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