Rapid Detection of Herpes Simplex Virus DNA in Genital Ulcers by Real-Time PCR Using SYBR Green I Dye as the Detection Signal

We have evaluated a real-time PCR procedure based on the LightCycler technology for rapid detection of herpes simplex virus (HSV) in genital lesions. Two sets of primers, corresponding to the thymidine kinase and DNA polymerase regions, were used for the amplification reactions in separate capillari...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Aldea, Carmen, Alvarez, Carmen P., Folgueira, Lola, Delgado, Rafael, Otero, Joaquín R.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2002
Assuntos:	Virology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC120289/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11880439/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.3.1060-1062.2002
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