Rapid Detection of Herpes Simplex Virus DNA in Genital Ulcers by Real-Time PCR Using SYBR Green I Dye as the Detection Signal

We have evaluated a real-time PCR procedure based on the LightCycler technology for rapid detection of herpes simplex virus (HSV) in genital lesions. Two sets of primers, corresponding to the thymidine kinase and DNA polymerase regions, were used for the amplification reactions in separate capillari...

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Veröffentlicht in:	J Clin Microbiol
Hauptverfasser:	Aldea, Carmen, Alvarez, Carmen P., Folgueira, Lola, Delgado, Rafael, Otero, Joaquín R.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2002
Schlagworte:	Virology
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC120289/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11880439/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.3.1060-1062.2002
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