Comparison of Real-Time, Quantitative PCR with Molecular Beacons to Nested PCR and Culture Methods for Detection of Mycobacterium avium subsp. paratuberculosis in Bovine Fecal Samples

An automated PCR with fluorescent probes (molecular beacons) detected Mycobacterium avium subsp. paratuberculosis in bovine feces. When the PCR was compared with culture in testing 41 fecal samples, kappa scores of 0.94 to 0.96, a sensitivity of 93 to 96%, and a specificity of 92% were obtained. Res...

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Detalhes bibliográficos

Publicado no:	J Clin Microbiol
Principais autores:	Fang, Ying, Wu, Wai-Hong, Pepper, Jessica L., Larsen, Jill L., Marras, Salvatore A. E., Nelson, Eric. A., Epperson, William B., Christopher-Hennings, Jane
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2002
Assuntos:	Clinical Veterinary Microbiology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC120117/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11773135/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.1.287-291.2002
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