Comparison of Real-Time, Quantitative PCR with Molecular Beacons to Nested PCR and Culture Methods for Detection of Mycobacterium avium subsp. paratuberculosis in Bovine Fecal Samples

An automated PCR with fluorescent probes (molecular beacons) detected Mycobacterium avium subsp. paratuberculosis in bovine feces. When the PCR was compared with culture in testing 41 fecal samples, kappa scores of 0.94 to 0.96, a sensitivity of 93 to 96%, and a specificity of 92% were obtained. Res...

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Publicado en:	J Clin Microbiol
Autores principales:	Fang, Ying, Wu, Wai-Hong, Pepper, Jessica L., Larsen, Jill L., Marras, Salvatore A. E., Nelson, Eric. A., Epperson, William B., Christopher-Hennings, Jane
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	American Society for Microbiology (ASM) 2002
Materias:	Clinical Veterinary Microbiology
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC120117/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11773135/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.1.287-291.2002
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