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Detection of Cryptococcus neoformans DNA in Tissue Samples by Nested and Real-Time PCR Assays
Two PCR protocols targeting the 18S rRNA gene of Cryptococcus neoformans were established, compared, and evaluated in murine cryptococcal meningitis. One protocol was designed as a nested PCR to be performed in conventional block thermal cyclers. The other protocol was designed as a quantitative sin...
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| Main Authors: | , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology
2002
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC119960/ https://ncbi.nlm.nih.gov/pubmed/11874894 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CDLI.9.2.461-469.2002 |
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