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Detection of Cryptococcus neoformans DNA in Tissue Samples by Nested and Real-Time PCR Assays

Two PCR protocols targeting the 18S rRNA gene of Cryptococcus neoformans were established, compared, and evaluated in murine cryptococcal meningitis. One protocol was designed as a nested PCR to be performed in conventional block thermal cyclers. The other protocol was designed as a quantitative sin...

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Dettagli Bibliografici
Autori principali: Bialek, Ralf, Weiss, Michael, Bekure-Nemariam, Kubrom, Najvar, Laura K., Alberdi, Maria B., Graybill, John R., Reischl, Udo
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2002
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC119960/
https://ncbi.nlm.nih.gov/pubmed/11874894
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CDLI.9.2.461-469.2002
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