Carregant...
A new method for determining the absolute molarity of solutions of trypsin and chymotrypsin by using p-nitrophenyl N(2)-acetyl-N(1)-benzylcarbazate
1. p-Nitrophenyl N(2)-acetyl-N(1)-benzylcarbazate (NPABC) was synthesized and shown to acylate α-chymotrypsin stoicheiometrically; reaction at 25° occurs almost instantaneously at pH7·04 and within 2min. at pH5·04 and there is no observable turnover during 10min. 2. The absolute molarity of solution...
Guardat en:
| Autors principals: | , |
|---|---|
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
1968
|
| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1198616/ https://ncbi.nlm.nih.gov/pubmed/5642613 |
| Etiquetes: |
Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!
|